We can chat about your project and make recommendations to get you the help you need to proceed. Not sure where to start? Just stop by NRB 933C. We have experience using Cell Profiler and Image analysis, as well as connections to many of the image analysis core facilities on campus. In this tutorial, you will see how to use the CLC Genomics Workbench to find primers for PCR amplification of a specific region. In addition, we can follow the R introductory course with advice tailored to your project, in consultations that can range from brief visits to yearlong support. We provide guidance on how to configure and use R, the main workhorse of immunogenomics, and how to develop project-specific analysis paths with RStudio. We have experience with clinical trial experimental design, differential expression analysis, as well as general statistics. General statistics advice and consulting services are available for help with experimental design or how to compare results from expression analysis. Seurat: We provide general consulting help on how to use Seurat We can help facilitate advice gathering from experts in the area.Ĭell Ranger: We have a Cell Ranger pipeline for department members This includes but is not limited to the Single Cell Genomics Core at BWH, as well as the Single Cell Core at HMS. On-bead tagmentation chemistry enables support for a wide range of DNA input amounts, various sample types, and a. 1 The workflow uses a single, 90-min hybridization step and as little as 10 ng input DNA. In addition, we have connections to single cell cores in the Longwood Medical Area. Our enrichment library prep yields provides > 90 on-target reads, > 95 uniformity, and low PCR duplicate rate across all Illumina sequencing systems. We provide advice and training support for both Cell Ranger and Seurat. We have a robust workflow and tutorial along with experts to help you through this process. The second option involves using CLC Genomics workbench ( Link) this is software package is installed on several of the machines in the IT suite (NRB933). For this option there are several ready built pipelines available for researchers to use. There are two major solutions that we have available: The first option involves using to the schools' computing cluster (O2) this would involve using software such as the Tuxedo Suite ( Link). We provide a number of solutions for analyzing RNA-seq data, and we are here to help provide guidance throughout this process.
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